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conditional gene knockout
Conditional gene knockout aka "conditional sequence deletion" or short: "conditional deletion", is a relatively new technique of genetic engineering, an offshoot of gene knockout technology where a specific target gene is eliminated from a single organ in the body of an experimental animal, rather than the whole body, as conventional gene knockout technology would entail. It can be applied to eukaryotic and prokaryotic systems. This allows for more sophisticated experiments.
== Technique ==
The most commonly used technique is using the Cre-Lox recombination system, described 1995. The enzyme Cre recombinase is a site-specific recombinase, which means it is promoting recombination by recognizing a short and specific nucleotide sequence without any added factor. Cre recombinase is known to be expressed in only a few cell types. Hence the researcher would be able to decide where exactly the gene in question is to be knocked out, thus allowing for a greater degree of freedom and scientific accuracy.
When publications are searched on Pubmed using the terms 'conditional sequence deletion' publication numbers have increased from initially in the teens per year during the late nineties to now well over 100 publications per year.〔http://www-ncbi-nlm-nih-gov/pubmed/?term=conditional+sequence+deletion〕
抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』
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